|
KMID : 0368419980410010009
|
|
Journal of Plant Biology
1998 Volume.41 No. 1 p.9 ~ p.15
|
|
|
Isolation and Characterization of o-Diphenol-O-Methyltransferase cDNA Clone in Hot Pepper(Capsicum annuum L.)
|
|
Lee, Byeong Ha
Choi, Dong Su/Lee, Kwang Woong
|
|
|
Abstract
|
|
|
|
A cDNA clone, CaOMT1 encoding an o-diphenol-O-methyltransferase (OMT), which is involved in capsaicin biosynthesis, was isolated by screening of a cDNA library prepared from the mRNA of pepper (Capsicum annuum L.) pericarp. Nucleotide sequence analysis of CaOMT1 revealed that it had an open reading frame of 1080 bp which encodes a polypeptide with a predicted molecular weight of 39,430 D, corresponding well with the size of the known OMT´s of tobacco, poplar, aspen, alfalfa, and cabbage. It also had five conserved boxes which appear in al known OMT´s. The nucleotide sequence of CaOMT1 had 89∼74% identity with the OMT cDNA´s of tobacco, aspen, alfalfa, and poplar, but a relatively lower identity of 59% with the OMT cDNA of maize. Amino acid sequence analysis also revealed that CaOMT1 has high identity with the known OMT´s which have a substrate of o-diphenolic compounds, especially 5-hydroxyferulic acid and caffeic acid. It supports CaOMT1 which encodes an OMT. Southern blot analysis suggested that CaOMT1 might exist in the form of multiple copies in the pepper genome. CaOMT1 is expressed preferentially in pepper fruit and its expression levels increased during pepper fruit development, but decreased during fruit ripening, suggesting that the CaOMT1 gene is fruit development-related. CaOMT1 is the first reported cDNA clone for enzymes related to the phenlypropanoid pathway in pepper.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|